| 1st | (Seed 3) Milwaukee Milkers |
| 2nd | (Seed 2) International Warriors |
| 3rd | (Seed 1) Chicago Bakla Bakla |
| 4th | (Seed 6) Charlotte Queen City Casual Sets |
| 5th | (Seed 11) Cincinnati Dig Pigs |
| 5th | (Seed 5) Chicago Reading Rainhoes |
| 7th | (Seed 10) STL Smash or Pass |
| 7th | (Seed 8) Pittsburgh Junk Company |
| 9th | (Seed 9) Pittsburgh Yellow Jackets |
| 9th | (Seed 15) Cleveland LEVA Hott Mess |
| 9th | (Seed 12) Cincinnati Dorothy's Friends |
| 9th | (Seed 4) Lexington Blue Horses |
| 13th | (Seed 14) Pittsburgh Girl Car |
| 13th | (Seed 13) Midwest Bossy Bitches |
| 13th | (Seed 7) Nashville Wrecking Ballz |
| 13th | (Seed 16) Pittsburgh Schweaty Balls |
| 17th | (Seed 17) Cincinnati The Kitten Heels |
| 17th | (Seed 18) Pittsburgh Dust Bunnies |
