| 1st | (Seed 6) PR Spartans |
| 2nd | (Seed 9) DC District Tips |
| 3rd | (Seed 7) Chicago Shady (Step)Sisters |
| 4th | (Seed 5) Chicago Pancakes |
| 5th | (Seed 2) Minneapolis ESHAS 2.8 |
| 5th | (Seed 20) Chicago Green Tea Girlies |
| 7th | (Seed 18) Milwaukee Spotted Cows |
| 7th | (Seed 1) Chicago Bakla Bakla |
| 9th | (Seed 3) Chicago NO MA'AM |
| 9th | (Seed 8) Manilla Pak Ganem |
| 9th | (Seed 19) Milwaukee Peaches |
| 9th | (Seed 15) Charlotte Queen City Casual Sets |
| 13th | (Seed 11) Chicago Reading Rainhoes |
| 13th | (Seed 10) Chicago Legally Blocked |
| 13th | (Seed 4) Chicago Gummy Yummy |
| 13th | (Seed 17) Chicago Not The Face |
| 17th | (Seed 16) Saint Paul Fuego |
| 17th | (Seed 24) Boston Gnarly |
| 17th | (Seed 13) Iowa City Buck Sets |
| 17th | (Seed 21) Chicago Daddy's Favourite |
| 17th | (Seed 12) Chicago Chicago Cuties |
| 17th | (Seed 26) STL/CHI Desperate Houseboys |
| 17th | (Seed 14) Boston T Party |
| 17th | (Seed 22) Boston You Can't Block This |
| 25th | (Seed 23) Chicago Volleywood Divas |
| 25th | (Seed 25) Chicago Podras Jamas |
