| 1st | (Seed 2) Chicago 2 Legit 2 Hit |
| 2nd | (Seed 1) Pyongyang K-Poppers |
| 3rd | (Seed 13) Minneapolis ESHAS STILL 2.5ish |
| 4th | (Seed 6) Milwaukee Spotted Cows |
| 5th | (Seed 3) Milwaukee Milkers |
| 5th | (Seed 5) Manila Barbie and the Kendolls |
| 7th | (Seed 10) STL XOXO - BARKADAZ |
| 7th | (Seed 8) Minneapolis Kinky Sets |
| 9th | (Seed 4) Bangkok Dok Tong |
| 9th | (Seed 9) Minnesota ComeBack |
| 9th | (Seed 11) Minneapolis Squirt Squad |
| 9th | (Seed 20) Omaha Fuzzy Ninjas |
| 13th | (Seed 19) Milwaukee Peaches |
| 13th | (Seed 18) Bay Area Chicken Wings |
| 13th | (Seed 15) Omaha Wrecking Ballz |
| 13th | (Seed 16) DC Bottoms Up |
| 17th | (Seed 17) Minneapolis Flaminghoes |
| 17th | (Seed 24) Minneapolis Not So Nice |
| 17th | (Seed 7) Minnesota CumBack |
| 17th | (Seed 21) Minneapolis Spike A Pose |
| 17th | (Seed 12) Chicago Overserved |
| 17th | (Seed 23) Minneapolis Spikeatondra |
| 17th | (Seed 14) Chicago Daddy's Favorites |
| 17th | (Seed 22) Milwaukee ChuPapi |
