| 1st | (Seed 1) Cincinnati BTS: BornToSpike |
| 2nd | (Seed 2) Pittsburgh Go, Toros! |
| 3rd | (Seed 8) Pittsburgh Jem and the Holograms |
| 4th | (Seed 6) Washington DC Destroy that pAss |
| 5th | (Seed 4) Chicago Ball Busters |
| 5th | (Seed 3) Chicago Bumpers |
| 7th | (Seed 11) Pittsburgh Wumbo |
| 7th | (Seed 9) Lake Michigan Lady Lampreys |
| 9th | (Seed 10) Cincinnati Cunning Stunts |
| 9th | (Seed 13) Lexington Love Handles |
| 9th | (Seed 7) Lexington Big Deal |
| 9th | (Seed 5) Orlando Warriors |
| 13th | (Seed 14) Cincinnati Gang Bang Gals Reborn |
| 13th | (Seed 17) TerraMar Mermaids |
| 13th | (Seed 15) Toronto Clarksons |
| 13th | (Seed 12) Berlin Die Geißböcke |
| 17th | (Seed 16) Springsboro Volleyball fiends |
